Oregano is a plant utilized as a spice in numerous regions of the world due to its characteristic sensory attributes of flavor, color, and aroma. The use of oregano. This study evaluated growth patterns of Mexican oregano (Lippia berlandieri) under salt stress and tested the hypothesis that this species is salt tolerant. J Food Prot. Dec;74(12) doi: / Antifungal activity evaluation of Mexican oregano (Lippia berlandieri Schauer).
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The main advantage of using EOs in a polymer matrix coating is a slow diffusion of the antimicrobial agent, leaving high concentrations of the active compound in contact with the food surface where contamination has occurred or is likely to occur, for a long period [ 20 ].
Natural antimicrobial compounds effectiveness depends on the type, genus, species, and strain of the target microorganism and some food intrinsic and extrinsic factors such as pH, temperature, water content, atmospheric composition, and initial microbial load, with pH being one of the most important factors due to antimicrobial dissociation [ 3 ]. Among them, carboxymethyl cellulose CMC and starch S are the most widespread and economic biomaterials.
Essential oils also have the additional effect of maintaining the quality of the food during storage, including sensorial, nutritional, and functional properties [ 45 ]. Therefore, future investigations should be conducted for the application of CMC and starch as edible coatings with MOEO in food to confirm their antibacterial effect lippai mechanical and sensory properties. Moreover, there are evidences that EOs incorporated to edible films can inhibit a large variety of food-borne pathogens [ 1315 berlandeiri 19 ].
Listeria monocytogenes and Staphylococcus aureus Fermi model biological parameters values at different concentrations of Mexican oregano Lippia berlandieri Schauer essential oil and pH values added to CMC and starch edible films. MOEO chemical analysis showed that the two major compounds were thymol 2.
Journal of Food Quality
Thus, adequate pH values can change the membrane permeability and disintegrate the outer membrane of Gram-negative bacteria releasing lipopolysaccharides. The aim of this work was to evaluate the effect of pH and Mexican oregano essential oil concentration added to CMC and S edible films on Listeria monocytogenes and Staphylococcus aureus.
Microbial modeling has allowed estimating key intrinsic factors as pH and MOEO concentration with the synergistic effect against two important food-borne pathogens. In addition, its chemical, physical, and functional characteristics make them suitable to add essential oils EOs as antimicrobials [ 10 — 12 ]. However, many reports mentioned that, for proper operation of active edible films, the optimal pH should be considered at which the antimicrobial agent may have a state of dissociation that allows better antimicrobial activity.
Moreover, these authors pointed out that EO effectiveness is influenced by the sensitivity of the microorganism.
Researchers around the world are investigating the use of essential oils to protect food from microbial growth. This is an open access article distributed under the Creative Commons Attribution Licensewhich permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Indexed in Science Citation Index Expanded. In this case, two main components of MOEO are thymol and carvacrol whose pH dissociation values are acidic 4. The data used to support the findings of this study are available from the corresponding author upon request. Carvacrol 5-isopropylmethylphenol liplia thymol 2-isopropylmethylphenol are the major components of the essential oils of some species belonging to the Lamiaceae family berlndieri oregano, and these are phenolic compounds; isomers of monoterpenes exhibit significant antimicrobial activity in vitro [ 67 ].
Edible Film Preparation Berlandierk were made according to the Bertuzzi et al. As observed, when MOEO concentration increased, a rapid microbial inactivation was observed, while at lower concentrations, the inactivation presented a remarkable nonlinear kinetics.
Inhibition curves of Listeria monocytogenes at different concentrations of Mexican oregano Lippia berlandieri Schauer essential oil and pH values added to CMC a 0. Subscribe to Table of Contents Alerts. Materials and Methods 2. San Claudio, Ciudad Universitaria, Col. Microbial modeling allows estimating the effects berlabdieri key intrinsic factors such as berladnieri on CMC and starch edible films added with MOEO, showing a synergistic effect between EO concentration and pH, so these films might have the potential to inhibit the surface growth of pathogenic microorganisms in food by being suitable carriers of EO in different food applications.
Introduction Researchers around the world are investigating the use of essential berlandleri to protect food from microbial growth. MOEO was incorporated at 0. Table of Contents Alerts. Correspondence should be addressed to Raul Avila-Sosa ; xm.
Neotropical Plant Portal – Lippia berlandieri
In this report, the kind of polymer chain and pH affects the release of antimicrobial MOEO compounds due to many factors such as electrostatic interactions, osmosis, structural changes, and environmental conditions [ 732 ]. Inhibition curves of Berlahdieri aureus at different concentrations of Mexican oregano Berlansieri berlandieri Schauer essential oil and pH values added to CMC a 0.
Based on their traditional use, these extracts obtained from plants are first recommended, due to their broad-spectrum antimicrobial action and low side effects treatments bdrlandieri 3 ]. Therefore, CMC releases more active compounds. Several works in the literature focus on the evaluation of the antibacterial effect of EOs and propose them as an interesting option to ensure the safety and quality of minimally processed foods and extend their shelf life [ 1314 ].
Currently, research has been focused on the use of edible films due to their safety regarding consumption and friendly relation with the environment, and coating materials being used currently include polysaccharides cellulose derivatives, starch, chitin, and gumsproteins soy, milk, gelatin, corn zein, and glutenberlandeiri lipids oils, waxes, and resins [ 9 ].
This model describes a sigmoid decay and generates two biological parameters and between pH and MOEO concentration Table 1. Journal of Berlanndieri Quality. Inhibition curves were fitted to the Fermi model. Antimicrobial assay was performed. One inconvenience of EO direct application on surfaces by dipping, powdering, or spraying is that antimicrobial compounds can be neutralized or diffuse rapidly from the surface into the product [ 30 ]. Films were made according to the Bertuzzi et al. Then, sorbitol Aldrich Chemical Co.
All tests were run in triplicate. Data were fitted to the Fermi model [ 23 ] from which the biological parameters and were estimated by nonlinear regression using the KaleidaGraph 3. The difference berlandierri CMC and starch polymers is the release of the antimicrobial compounds mainly thymol and carvacrol present in MOEO. Moreover, the Fermi model can explain and predict microbial reduction beerlandieri all tested pathogens and is suitable to describe inactivation in similar matrices and under different operating conditions in the future [ 26 ].